Photoacoustic imaging of tumor vascular involvement and surgical margin pathology for feedback-guided intraoperative tumor resection

Surgery represents the mainstream therapeutic modality in oncology. Aggressive radical surgery to achieve no residual tumor would improve survival, which is mainly affected by vascular involvement and accuracy of judging negative margin resection. However, there currently intraoperative tool that can simultaneously perform microscopic analysis peritumoral vasculature vivo surgical pathology ex vivo, leads randomness one-time complete resection tumor, patient may have undergo secondary surgery. To address this critical need, we developed a 532/266 nm dual-wavelength photoacoustic (PA) microscopy imaging (532/266-PAI) system enables both regional pathological assessment fresh samples. A mammary animal model was established mimic process resection, from judgment margins. It proved 532/266-PAI technology identify through visualization, determine plan, then judge whether completely removed ultraviolet PA (UPA) imaging. Re-excision evaluation are performed when positive diagnosed intraoperation UPA The 266/532-PAI technique has great potential for navigation.